primary antibodies against p p65  (Bioss)

Journal: Pharmaceuticals

Article Title: UHPLC–Q–Orbitrap–HRMS-Based Multilayer Mapping of the Pharmacodynamic Substance Basis and Mechanistic Landscape of Maizibizi Wan in Chronic Nonbacterial Prostatitis Therapy

doi: 10.3390/ph19010153

Figure Lengend Snippet: MZBZ regulates key target protein expression. Western blot image of ( A ) P-p65/p65, ( B ) p-AKT/AKT, ( C ) BCL2, COX-2, and TGF-β1 protein expression.

Article Snippet: Antibodies and kits included a BCA protein assay kit (Cat. No. WLA004, Wanleibio, Shenyang, China) and primary antibodies against p-NF-κB p65 (WL02169), NF-κB p65 (WL01980), BCL2 (WL01556), COX-2 (WL01750), AKT1 (WL01652), p-AKT (WLP001), TGF-β1 (WL02193), and β-actin (WL01372), together with HRP-conjugated goat anti-rabbit IgG (WLA023; all from Wanleibio, Shenyang, Chnia).

Techniques: Expressing, Western Blot

Journal: Advanced Science

Article Title: Reprogramming Lung Redox Homeostasis by NIR Driven Ultra‐Small Pd Loaded Covalent Organic Framework Inhibits NF‐κB Pathway for Acute Lung Injury Immunotherapy

doi: 10.1002/advs.202413697

Figure Lengend Snippet: A) Relative genes expression levels of treated cells by RT‐qPCR: IL‐6 (i), IL‐1β (ii), SOD2 (iii), iNOS (iv), CD86 (v), CD206 (vi), IL‐10 (vii), HSP70 (viii), and CD31 (ix). B) iNOS expression level of treated cells by fluorescent microscope and the corresponding quantified results (C). D) CD206 expression level of treated cells by fluorescent microscope and the corresponding quantified results (E). F) HSP70 expression level of treated cells by fluorescent microscope and the corresponding quantified results (G). H) CD31 expression level of treated cells by fluorescent microscope and the corresponding quantified results (I). J) A heatmap of DEGs between control group and TP@Pd+NIR. K) A volcano plotting of the landscape of DEGs. L) A gene interaction network of the relationship between NF‐κB pathway related genes (Red node) and three genes of interest (Blue node). M) A correlation heatmap of the correlation between IκBα/p65 and three genes of interest. N) Relative proteins expression levels of treated cells by WB. The corresponding groups were: cells without treatment (normal group), cells pre‐treated with LPS followed by incubating with PBS (control group), cells pre‐treated with LPS followed by incubating with 200 µg mL −1 TP (TP), cells pre‐treated with LPS followed by incubating with 200 µg mL −1 TP@Pd (TP@Pd), and cells pre‐treated with LPS followed by incubating with 200 µg mL −1 TP@Pd and NIR irradiation (1 W cm −2 ) (TP@Pd+NIR). (“*” symbol compared with normal group, * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001).

Article Snippet: They were then incubated overnight at 4 °C with primary antibodies against IκBα, p‐IκBα, p65, or p‐p65 (Proteintech, China).

Techniques: Expressing, Quantitative RT-PCR, Microscopy, Control, Irradiation